Anti-Human CD5 Recombinant Antibody (5D7)
CAT#: TAB-308LC
This product is a recombinant monoclonal antibody against CD5. It binds to human and cynomolgus CD5. It is a mouse monoclonal antibody that can potentially be used to treat autoimmune diseases.
Published Data
Gene Expression
Tested Data
FC
Figure 1 Flow cytometry measuring binding of hybridoma-produced 5D7 to human and cynomolgus monkey PBMCs.
Cyt
Figure 2 ADCC assay with WT and variant anti-CD5 antibodies against Wac3CD5 target cells, using purified NK cells as effector cells. Anti-RSV IgGI was used as an isotype control.
Cyt
Figure 3 ADCC activity of humanized anti-CD5 antibodies against Wac3CD5 CLL target cells. All antibodies comprise the heavy chain constant region of Hybrid_S239D/l332E.
FuncS
Figure 4 Caspase activity assay demonstrating capacity of anti-CD5 antibody to mediate apoptosis against CLL cells.
Cyt
Figure 5 show ADCC activity of anti-CD5 antibodies against lymphoma and leukemia cell lines.
FuncS
Figure 6 In vitro depletion of T-cells in PBMCs by humanized anti-CD5 antibodies.
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Normal Tissue
Figure 1 Testis
Spermatogonia cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43416/101306_A_5_6.jpg
Normal Tissue
Figure 2 Lymph node
Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43416/101306_A_7_8.jpg
Normal Tissue
Figure 3 Tonsil
Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43416/101306_A_5_8.jpg
RNA Expression
Figure 4 RNA cell line category: Group enriched (JURKAT, MOLT-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000110448-CD5
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SDS-PAGE
Figure 1 Anti-Human CD5 Recombinant Antibody (TAB-308LC) in SDS-PAGE
SDS-PAGE analysis of TAB-308LC in β-mercaptoethanol-reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as about 180-275 kDa.
SEC-HPLC
Figure 2 Anti-Human CD5 Recombinant Antibody (TAB-308LC) in SEC-HPLC
The purity of TAB-308LC was greater than 98% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl
DB
Figure 3 Anti-Human CD5 Recombinant Antibody (TAB-308LC) in DB
Dot Blot analysis of TAB-308LC was performed by coating with human CD5 protein (His tag).
TAB-308LC incubation concentration: 2 μg/mL.
HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000)
WB
Figure 4 Anti-Human CD5 Recombinant Antibody (TAB-308LC) in WB
Western blot analysis of TAB-308LC was performed by loading human CD5 protein, His tag.
TAB-308LC incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat Anti-Mouse IgG (H+L).
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 2: Reducing Antigen (0.6 μg)
ELISA
Figure 5 Anti-Human CD5 Recombinant Antibody (TAB-308LC) in ELISA
ELISA analysis of TAB-308LC was performed by coating with human CD5 protein (His tag). Then blocked with BSA and incubated with TAB-308LC. The HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
FACS
Figure 6 Anti-Human CD5 Recombinant Antibody (TAB-308LC) in FACS
Flow cytometry analysis of hPBMC cells incubated with Anti-Human CD5 Recombinant Antibody, Cat# TAB-308LC. hPBMC cells were stained with TAB-308LC (blue histogram, TE232276-1) or isotype control antibody (yellow histogram), followed by FITC-conjugated goat anti-Mouse IgG secondary antibody. The used concentration of TAB-308LC was 5 µg per 5 x 10^6 cells.
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Specifications
- Host Species
- Mouse
- Specificity
- Human
- Species Reactivity
- Cynomolgus
- Clone
- 5D7
- Applications
- Cyt, FC, FuncS
- Related Disease
- Autoimmune diseases
Applications
- Application Notes
- This antibody has been reported for use in Cytotoxicity, Flow Cytometry, Functional Assay.
Target
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greater affinities and sophistication
The affinity-purified nature enabled strong and accurate binding, increasing the trustworthiness of the results in a variety of applications.
Flexible with Different Detergents
Even in the presence of mild detergents used in membrane protein studies, it performed consistently.
No Issues Regarding Aggregation
Even after extended storage, no aggregation was seen. Assures dependability and consistent results.
Q&As
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What purpose does an ELISA test normalizer fulfill?
A: The homogeneous assay conditions maintained by a normalizer help to compensate for well-to-well variability.
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Is an antibody suitable for multiplex testing?
A: It depends on how well the antibodies and detecting technology of the multiplex assay cooperate.
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How can I ensure the specificity of my antibody?
A: Use controls such as no-primary controls, isotype controls, and peptide blocking studies.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-308LC, RRID: AB_3111909)
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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- CAT
- Product Name
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|---|---|---|---|
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| TAB-885 | Mouse Anti-CD5 Recombinant Antibody (clone Telimomab); Fab Fragment | FuncS, IF, Neut, ELISA, FC, IP, ICC | Mouse Fab |
| TAB-308LC-S(P) | Anti-Human CD5 Recombinant Antibody scFv Fragment (5D7) | FC, SPR | |
| TAB-308LC-F(E) | Anti-Human CD5 Recombinant Antibody Fab Fragment (5D7) | FC, SPR |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-L052G | anti-CD5 immunotoxin HB21 (IgG)-Gel | Cytotoxicity assay, Functional assay | |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-309LC | Anti-Human CD5 Recombinant Antibody (H3L1) | FC, SPR | Chimeric antibody (mouse/human) |
| TAB-310LC | Anti-Human CD5 Recombinant Antibody | WB | Chimeric antibody (mouse/human) |
| TAB-309LC-S(P) | Anti-Human CD5 Recombinant Antibody scFv Fragment (H3L1) | FC, SPR | Chimeric antibody (mouse/human) |
| TAB-309LC-F(E) | Anti-Human CD5 Recombinant Antibody Fab Fragment (H3L1) | FC, SPR | Chimeric antibody (mouse/human) |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-392CQ | Rat Anti-CD5 Recombinant Antibody (clone 53-7.3) | FC, IHC-Fr, IP, ELISA, Block | Rat IgG2a, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0567 | Hi-Affi™ Rabbit Anti-CD5 Recombinant Antibody (clone DS567AB) | IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| HPAB-0779-WJ-S(P) | Mouse Anti-CD5 Recombinant Antibody (clone 1D8); scFv Fragment | ELISA, WB | Mouse scFv |
| HPAB-0780-WJ-S(P) | Mouse Anti-CD5 Recombinant Antibody (clone 3I21); scFv Fragment | ELISA, WB | Mouse scFv |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| HPAB-0780-WJ | Human Anti-CD5 Recombinant Antibody (clone 3I21) | ELISA, WB | Chimeric (mouse/human) IgG |
| HPAB-0781-WJ | Human Anti-CD5 Recombinant Antibody (clone 4H10) | ELISA, WB | Chimeric (mouse/human) IgG |
| HPAB-0782-WJ | Human Anti-CD5 Recombinant Antibody (clone 8J23) | ELISA, WB | Chimeric (mouse/human) IgG |
| HPAB-0783-WJ | Human Anti-CD5 Recombinant Antibody (clone 5O4) | ELISA, WB | Chimeric (mouse/human) IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0779-WJ-F(E) | Human Anti-CD5 Recombinant Antibody (clone 1D8); Fab Fragment | ELISA, WB | Chimeric (mouse/human) Fab |
| HPAB-0780-WJ-F(E) | Human Anti-CD5 Recombinant Antibody (clone 3I21); Fab Fragment | ELISA, WB | Chimeric (mouse/human) Fab |
| HPAB-0781-WJ-F(E) | Human Anti-CD5 Recombinant Antibody (clone 4H10); Fab Fragment | ELISA, WB | Chimeric (mouse/human) Fab |
| HPAB-0782-WJ-F(E) | Human Anti-CD5 Recombinant Antibody (clone 8J23); Fab Fragment | ELISA, WB | Chimeric (mouse/human) Fab |
| HPAB-0783-WJ-F(E) | Human Anti-CD5 Recombinant Antibody (clone 5O4); Fab Fragment | ELISA, WB | Chimeric (mouse/human) Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC924 | AbPlus™ Anti-CD5 Magnetic Beads (VS-0724-YC924) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY58 | CytoStream™ Mouse Anti-CD5 Recombinant Antibody (clone OX-19) | FC | Mouse IgG1, kappa |
| VS-0225-XY59 | CytoStream™ Mouse Anti-CD5 Recombinant Antibody (clone UCHT2) | FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1195 | Anti-CD5 Immunohistochemistry Kit | IHC | |
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